Steven A. Ripp

Research Associate Professor - Center for Environmental Biotechnology

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Dr. Ripp earned a bachelor’s degree in Microbiology from the University of Wisconsin-Madison and a Ph.D. in Microbiology and Molecular Biology from Oklahoma State University. He completed his postdoctoral training at the University of Tennessee/Oak Ridge National Laboratory under an Alexander Hollaender Fellowship and currently serves as a Research Associate Professor at UT. His research interests emphasize the integration of synthetic biology, molecular biology, and bioelectronics to create cellular reporter systems for biomedical imaging with ancillary applications in environmental monitoring.

Focus Areas:  Biomedical Imaging | Bioreporters | Biosensors | Bioelectronics

Skills and Expertise:  Synthetic Biology | Recombinant Expression Systems | Bioluminescent and Fluorescent Reporter Gene Fusions | Microbiology | Molecular Biology

Selected Publications:

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
T. Xu, D. Close, S. Webb, S. Ripp, and G. Sayler, The Journal of Visualized Experiments, (in press),
Expression of Non-Native Genes In A Surrogate Host Organism
D. Close, T. Xu, A. Smartt, S. Price, S. Ripp, and G. Sayler, In Barrera-Saldana HA (ed.), Genetic Engineering - Basics, New Applications and Responsibilities. Intech Publishers, Rijeka, Croatia., pp. 3-34, 2012.
Silicon Photomultiplier (SPM) Detection of Low-Level Bioluminescence for the Development of Deployable Whole-Cell Biosensors: Possibilities and Limitations
H. Li, N. Lopes, S. Moser, G. Sayler, and S. Ripp, Biosensors and Bioelectronics, vol. 33, no. 1, pp. 299-303, 2012.
Applications of Bacteriophages for the Control of Veterinary Pathogens
M. Prado, D. Ghosh, and S. Ripp, In Boulanger A, Blanc M (ed.), Bacterial Pathogens: Virulence Mechanisms, Diagnosis and Management. Nova Science Publishers, Inc., Hauppauge, NY.., 2012.
Pathogen Detection Using Engineered Bacteriophages
A.E. Smartt, X. Tingting, P. Jegier, J.J. Carswell, S.A. Blount, G.S. Sayler, and S. Ripp, Analytical and Bioanalytical Chemistry, vol. 402, no. 10, pp. 3127-3146, 2011.
Comparison of Human Optimized Bacterial Luciferase, Firefly Luciferase, and Green Fluorescent Protein for Continuous Imaging of Cell Culture and Animal Models
D.M. Close, R.E. Hahn, S.S. Patterson, S.J. Baek, S.A. Ripp, and G.S. Sayler, Journal of Biomedical Optics, vol. 16, no. 4, 2011.
In Vivo Bioluminescent Imaging (BLI): Noninvasive Visualization and Interrogation of Biological Processes in Living Animals
D.M. Close, X. Tingting, G.S. Sayler, and S. Ripp, Sensors, vol. 11, no. 1, pp. 180-206, 2011.
Autonomous Bioluminescent Expression of the Bacterial Luciferase Gene Cassette (lux) in a Mammalian Cell Line
D.M. Close, S.S. Patterson, S. Ripp, S.J. Baek, J. Sanseverino, and G.S. Sayler, PLoS ONE, 5:e12441, 2010.
Bioelectronics
J.T. Fleming and S. Ripp, In M.C. Flickinger (ed.), Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, John Wiley & Sons, Hoboken, NJ, 2010.
Reporter Proteins in Whole-Cell Optical Bioreporter Detection Systems, Biosensor Integrations, and Biosensing Applications
D.M. Close, S. Ripp, and G.S. Sayler, Sensors, vol. 9, no. 11, pp. 9147-9174, 2009.