The Waters GPC system consists of a Waters model 510 pump, a Rheodyne model 7725(i) manual injector with a 200 ul loop, and a Knauer Smartline model 2300 RI detector. For applications in DMF or DMF+0.1M LiBr, the following column set is used: four PSS (Polymer Standards Service) GRAM; 8×300 mm; 10µm; 100, 1000, and 3000 Å along with an 8×50, 10µm guard. For applications in toluene or chloroform, the following column set is used: one Jordi Gel DVB; 10×250 mm; 5µm; 500 Å, and two Jordi Gel DVB; 10×250 mm; 5µm; mixed bed. Both sets of columns are calibrated with a set of PS standards in the molecular weight range of ≈600 up to 7,500,000 daltons.
- Column set: PSS (Polymer Standards Service) GRAM; 8×300 mm; 10μm; 100, 1000, and 3000 Å along with an 8×50, 10μm guard
- Temperature: room temperature, ≈25°C
- Solvent: N,N-dimethylformamide + 0.1 M LiBr