The aqueous GPC system consists of a Waters model 510 pump, a Waters model U6K injector, and a Waters model R401 differential refractometer. Data is collected and analyzed using Polymer Labs Cirrus software. The column set installed in this instrument consists of the following four columns: PSS (Polymer Standards Service) suprema; 8×300 mm; 10 µm; 100, 3000, and 10,000 Å along with an 8×50, 10µm guard. The instrument is calibrated in the molecular weight range of ≈600 up to 1,000,000 Daltons using a set of PEO/PEG standards. The following additional set of four columns is available for aqueous applications: three Polymer Laboratories PLaquagel-OH; 7.5×300 mm; 8 µm; MIXED along with a 7.5×50, 8µm guard.
- Column set: PSS (Polymer Standards Service) suprema; 8×300 mm; 10 μm; 100, 3000, and 10,000 Å along with an 8×50, 10 μm guard
- Temperature: room temperature ≈25°C
- Solvent: water + 0.02%NaN3